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KMID : 0363219720100010011
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Korean Journal of Dermatology
1972 Volume.10 No. 1 p.11 ~ p.22
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Experimental Study on Tissue Mast Cell Change of Ratskin Elicited by Mechanical Irritation Under General and Local Anesthesia
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Yoo Tae-Yun
Kim Hong-Sig
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Abstract
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Normal albino rats weighing 180-200 gm were used for a series of this investigation on change of skin mast cell under general and local anesthesia. At first, left side abdominal skin was prepared by hair cutting with scissors about 3cm square and eight irritations by mean of biting with tip of forceps mosquito were applied along the prepared skin margin. Skin specimens were submitted from central area which surrounded by irritated points and also from intact contra-lateral area in 15, 30, 60 and 120 minutes invervals. Submitted skin specimens were fixed in 10% formalin solution, then were stained with 1% toluidine blue. The number of mast cell was counted under 45OX of microscopic examination.
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